Anti-MeCP2 antibody ab2828 from Abcam

Product Specs
ItemAnti-MeCP2 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Supplier Product Page
Catalog Numberab2828
Antibody TypePrimary
ClonalityPolyclonal
Quantity50 UG
IsotypeIgG
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameMECP2
ReactivityHuman, Mouse, Rat
TargetMeCP2

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Kamishoyosan and Kamikihito protect against decreased KCC2 expression induced by the P. gingivalis lipopolysaccharide treatment in PC-12 cells and improve behavioral abnormalities in male miceHeliyonDecember 1, 2023Kazuo Tomita, Yukiko Oohara, Kento Igarashi, Junichi Kitanaka, Nobue Kitanaka, Koh-ichi Tanaka, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Mitsutaka Sugimura, Tomoaki Satoanti-pSPAK (Ser373): 07-2273, Merck, KGaA, Darmstadt, Germany; anti-KCC2: AB3560P, Merck; anti- MECP2: ab2828, Abcam, Cambridge, UK; anti-REST: bs-2590R, Bios Antibodies Inc. Woburn, MA, USA). Secondary antibodies

(2) A novel pathogenic mutation of MeCP2 impairs chromatin association independent of protein levelsGenes & DevelopmentOctober 1, 2023Jian Zhou, Claudia Cattoglio, Yingyao Shao, Harini P. Tirumala, Carlo Vetralla, Sameer S. Bajikar, Yan Li, Hu Chen, Qi Wang, Zhenyu Wu, et al.was incubated overnight at 4°C with anti-MeCP2 antibody per the manufacturer's recommendation (Abcam ab2828). An aliquot of 10% of the precipitated chromatin was stored as input. The next day, 40 μL of Protein

(3) DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loopsCell reportsMarch 28, 2023Marchena-Cruz E, Camino LP, Bhandari J, Silva S, Marqueta-Gracia JJ, Amdeen SA, Guillén-Mendoza C, García-Rubio ML, Calderón-Montaño JM, Xue X, et al.AB_260634 Mouse anti-IgG,Sigma,Cat# I8765;RRID: AB_1163672 Polyclonal antibody Rabbit anti- MECP2,Abcam,Cat# ab2828;RRID: AB_2143853 Polyclonal antibody Rabbit anti-MECP2 ChIP Grade,Abcam,Cat# ab195393;RRID: AB_2848209 Polyclonal

(4) The HP1α protein is mandatory to repress the circadian clock and its output genes during the 12 h period of transcriptional repressionProceedings of the National Academy of SciencesFebruary 21, 2023Nisha Misra, Manohar Damara, Pierre Chambonalpha (TDGα) (Active Motif 61437), DNMT3a (Abcam ab13888), MDB4 (Santacruz sc-398249X), MECP2 (Abcam ab2828), H3K9Ac (Abcam ab4441), H3K9Me3 (Abcam ab8898), YY1 (Abcam ab38422), H3K4Me1 (Active motif 39297),

(5) Regulation of BDNF transcription by Nrf2 and MeCP2 ameliorates MPTP-induced neurotoxicityCell Death DiscoveryDecember 1, 2022Qianqian Cao et al.β-actin or GAPDH loading controls. Antibodies against Nrf2 (ab137550), BDNF (ab108319), and MeCP2 (ab2828) were purchased from Abcam, BDNF (47808) was purchased from Cell Signaling Technology (CST), MeCP2 (M6818)

(6) Modeling RTT Syndrome by iPSC-Derived Neurons from Male and Female Patients with Heterogeneously Severe Hot-Spot MECP2 VariantsInternational Journal of Molecular SciencesNovember 21, 2022Sara Perego, Valentina Alari, Gianluca Pietra, Andrea Lamperti, Alessandro Vimercati, Nicole Camporeale, Maria Garzo, Francesca Cogliati, Donatella Milani, Aglaia Vignoli, et al.identify neuronal cells, while Anti-MeCP2 C-ter (1:100, ab2829, Abcam) and Anti MeCP2 N-ter (1:40, ab2828, Abcam) were used to discriminate different portions of MeCP2 protein. Secondary antibodies Goat Anti-Mouse-IgG (H + L

(7) MeCP2 inhibits ischemic neuronal injury by enhancing methylation of the FOXO3a promoter to repress the SPRY2-ZEB1 axisExperimental & molecular medicineAugust 1, 2022Meng L, Feng B, Luan L, Fang Z, Zhao G.Thermo Fisher Scientific, USA) was employed with rabbit polyclonal antibody against MeCP2 (ChIP Grade) (ab2828, Abcam, Inc., Cambridge, UK), mouse monoclonal antibody against histone H3K9me2 (ChIP Grade) (ab1220,

(8) 4,8-dicarboxyl-8,9-iridoid-1-glycoside Promotes Neural Stem Cell Differentiation Through MeCP2Dose-response : a publication of International Hormesis SocietyJuly 1, 2022Wang W, Liu Z, Jing B, Mai H, Jiao H, Guan T, Chen D, Kong J, Pan T.were as follows: Antibodies: Anti-MAP2 antibody: Abcam Cat# ab28032; Anti-MeCP2 antibody: Abcam Cat# ab2828; Anti-PDGFR-alpha: Abcam Cat# ab96569; Anti-Myelin Basic Protein antibody: Abcam Cat# ab218011; Anti-GFAP

(9) Novel role of UHRF1 in the epigenetic repression of the latent HIV-1eBioMedicineMay 1, 2022Roxane Verdikt et al.EMSA and western blotting (MABE308) were purchased from Upstate/Millipore. Antibodies against MeCP2 (ab2828, RRID:AB_2143853) and RBP-JK (ab33065, RRID:AB_778156) were purchased from Abcam. Antibody against RNA

(10) MeCP2 Epigenetic Silencing of Oprm1 Gene in Primary Sensory Neurons Under Neuropathic Pain ConditionsFrontiers in NeuroscienceNovember 5, 2021Na Sun et al.sample. Chromatin was added with protein G magnetic beads plus anti-MeCP2 antibody (1:20, Abcam, Cat No: ab2828, RRID:AB_2143853), anti-HDAC1 antibody (1:20, Abcam, Cat No: ab19845, RRID:AB_470299), or anti-HDAC2

(11) MeCP2 duplication causes hyperandrogenism by upregulating LHCGR and downregulating RORαCell death & diseaseOctober 25, 2021Wang YM, Wu Y, Zheng YF, Wang HY.protein-DNA complexes were immunoprecipitated by incubating with rabbit anti-MeCP2 antibody (Abcam, Cat# ab2828), rabbit anti-CREB antibody (Cell Signaling, Cat# 9197 S), anti-DNA G-quadruplex structures antibody, clone BG4

(12) Activation of BDNF by transcription factor Nrf2 contributes to antidepressant-like actions in rodentsTranslational PsychiatryJune 1, 2021Wei Yao, Song Lin, Jin Su, Qianqian Cao, Yueyue Chen, Jiaxu Chen, Zhentao Zhang, Kenji Hashimoto, Qi Qi, Ji-chun Zhangpurchased from Abcam: Nrf2 (ab137550), BDNF (ab108319), HDAC2 (ab12169), mSin3A (ab3479), and MeCP2 (ab2828). The beta-actin antibody was purchased from EarthOx. On the day of injection, fresh solutions were

(13) Epigenetic regulation of p62/SQSTM1 overcomes the radioresistance of head and neck cancer cells via autophagy-dependent senescence inductionCell Death & DiseaseMarch 5, 2021Myungjin Lee et al.(07-352; Sigma-Aldrich), Histone H3K4me3 (39915; Active Motif), Histone H3K27me3 (ab6002; Abcam), MeCP2 (ab2828; Abcam), and β-actin (A5441; Sigma). Chromatin immunoprecipitation (ChIP) assay A ChIP assay was performed

(14) Heritable pattern of oxidized DNA base repair coincides with pre-targeting of repair complexes to open chromatinNucleic acids researchJanuary 11, 2021Bacolla A, Sengupta S, Ye Z, Yang C, Mitra J, De-Paula RB, Hegde ML, Ahmed Z, Mort M, Cooper DN, et al.α-H4K16Ac (Cell Signaling; #13534), anti-G-quadruplex DNA 1H6 (Millipore; #MABE1126), α-MeCP2 (AbCam; #ab2828), α-Pan-Methyl-H3K9 (Cell Signaling; #4473), α-methyl-histone H3K9 (Cell Signaling; #5327), α-RNA Pol

(15) Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatmentCancer Cell InternationalDecember 1, 2020Siyuan Chang, Jing Huang, Huan Niu, Jing Wang, Yang Si, Zhigang Bai, Shan Cheng, Wei DingSanta Cruz Biotechnology, Dallas, Texas, USA) were loaded either with the following ChIP-validated anti-MeCP2 (ab2828, Abcam, Cambridge, UK) antibody or control IgG (2729, Cell Signaling Technology). The beads were incubated

(16) MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcriptionOncogenesisJune 1, 2020Tong D, Zhang J, Wang X, Li Q, Liu LY, Yang J, Guo B, Ni L, Zhao L, Huang C.immunoprecipitated with Dynal magnetic beads (Invitrogen, Carlsbad, CA, USA) and antibodies for MeCP2 (ab2828, Abcam, Cambridge, MA, USA) or GFP (ab290, Abcam). After DNA elution and purification, qPCR and/or PCR

(17) Redefining transcriptional regulation of the APOE gene and its association with Alzheimer’s diseasePLOS ONEJanuary 24, 2020Eun-Gyung Lee et al.immunoprecipitation, we incubated 1 μg of chromatin with 0.8 μg of rabbit polyclonal anti-MECP2 antibody (ab2828, Abcam) overnight. We then purified protein-enriched DNA fragments using a Magnetic Onestep Kit (Abcam)

(18) Comprehensive Analysis of GABAA-A1R Developmental Alterations in Rett Syndrome: Setting the Focus for Therapeutic Targets in the Time Frame of the DiseaseInternational Journal of Molecular SciencesJanuary 14, 2020Alfonso Oyarzabal et al.was directed against GABA-A1 (Neuromab, UCDavis, CA, USA, 75-136) at a concentration of 1:500, MeCP2 (ab2828; Abcam, Madrid, Spain) at a concentration of 1:1000, and vinculin (ab129002, Abcam, Madrid, Spain) was

(19) Altered amygdala DNA methylation mechanisms after adolescent alcohol exposure contribute to adult anxiety and alcohol drinkingNeuropharmacologyOctober 1, 2019Amul J. Sakharkar, Evan J. Kyzar, David P. Gavin, Huaibo Zhang, Ying Chen, Harish R. Krishnan, Dennis R. Grayson, Subhash C. Pandeyfrom each sample were separated for chromatin immunoprecipitation using an antibody against MeCP2 (ab2828; Abcam, Cambridge, MA, USA) and for quantifying the amount of DNA in different samples before immunoprecipitation

(20) MeCP2 inhibits cell functionality through FoxO3a and autophagy in endothelial progenitor cellsAging (Albany NY)September 2, 2019Siyuan Zha, Zhen Li, Shuyan Chen, Fang Liu, Fei WangThermo Fisher Scientific. Treated cells were fixed with 1% formaldehyde and sheared by sonication. MeCP2 antibody (ab2828; Abcam, Cambridge, UK) was used to www.aging-us.com 6726 immunoprecipitate the MeCP2-DNA complex

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Anti-MeCP2 antibody from Abcam

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Anti-MeCP2 antibody from Abcam

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